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Registro Completo |
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Biblioteca(s): |
Embrapa Meio-Norte. |
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Data corrente: |
05/09/1997 |
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Data da última atualização: |
11/12/2009 |
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Autoria: |
GUIMARAES, P. M. |
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Título: |
Development of a DNA probe and characterisation of Curtobacterium flaccumfaciens pv. flaccumfaciens. |
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Ano de publicação: |
1997 |
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Fonte/Imprenta: |
London: University of London, 1997. |
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Páginas: |
224p. |
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Idioma: |
Inglês |
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Notas: |
Ph.D. Thesis. |
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Conteúdo: |
Bacterial wilt of beans is a seedborne disease caused by the plant pathogenic coryneform bacteria Curtobacterium flaccumfaciens pv. flaccumfaciens. In order to construct a DNA probel for the identification of this organism, a chromosomal DNA library was built in the plasmid vector pGEM-7zf(+). The "Shot-gun" method was used for the construction of the DNA library in conjunction with a differential hybridisation technique. One of the clones (pPMP-26 D), after subsequent digestion with restriction endonucleases EcoRI/SacI, yielded a fragment of approximately 230 bp (pPMP-26 D) which hybridised specifically to C. f.pv. flaccumfaciens and not to any of the other eight-two coryneforms or bean pathogens tested in Southern, colony and dot-blots. Thisfragment was subcloned for use as a probe for the detection and identification of this organism. The uniqueness of the probe was confirmed by DNA sequencing. In addition, chemotaxonomic (fatty acid analysis) and genomic fingerpriting technique (ITS profiling, PCR of repetitive DNA and macrorestriction analysis by PFGE) were examined for the identification of profiles which allowed the differentiation of the pathovarswithin the species. The most discriminatory results were obtained by the use of pulsed-field gel electrophoresis analysis of HindIII and XbaI digests, Which produced markedly different patterns for each of the four C. |
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Palavras-Chave: |
Bean; Germoplasma: Patogeno: Identificação; Identifications. |
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Thesagro: |
Feijão; Phaseolus Vulgaris. |
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Thesaurus Nal: |
Curtobacterium flaccumfaciens; germplasm. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02023nam a2200217 a 4500
001 1051987
005 2009-12-11
008 1997 bl uuuu m 00u1 u #d
100 1 $aGUIMARAES, P. M.
245 $aDevelopment of a DNA probe and characterisation of Curtobacterium flaccumfaciens pv. flaccumfaciens.
260 $aLondon: University of London$c1997
300 $a224p.
500 $aPh.D. Thesis.
520 $aBacterial wilt of beans is a seedborne disease caused by the plant pathogenic coryneform bacteria Curtobacterium flaccumfaciens pv. flaccumfaciens. In order to construct a DNA probel for the identification of this organism, a chromosomal DNA library was built in the plasmid vector pGEM-7zf(+). The "Shot-gun" method was used for the construction of the DNA library in conjunction with a differential hybridisation technique. One of the clones (pPMP-26 D), after subsequent digestion with restriction endonucleases EcoRI/SacI, yielded a fragment of approximately 230 bp (pPMP-26 D) which hybridised specifically to C. f.pv. flaccumfaciens and not to any of the other eight-two coryneforms or bean pathogens tested in Southern, colony and dot-blots. Thisfragment was subcloned for use as a probe for the detection and identification of this organism. The uniqueness of the probe was confirmed by DNA sequencing. In addition, chemotaxonomic (fatty acid analysis) and genomic fingerpriting technique (ITS profiling, PCR of repetitive DNA and macrorestriction analysis by PFGE) were examined for the identification of profiles which allowed the differentiation of the pathovarswithin the species. The most discriminatory results were obtained by the use of pulsed-field gel electrophoresis analysis of HindIII and XbaI digests, Which produced markedly different patterns for each of the four C.
650 $aCurtobacterium flaccumfaciens
650 $agermplasm
650 $aFeijão
650 $aPhaseolus Vulgaris
653 $aBean
653 $aGermoplasma: Patogeno: Identificação
653 $aIdentifications
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Registro original: |
Embrapa Meio-Norte (CPAMN) |
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| Registros recuperados : 279 | |
| 3. |  | GUIMARAES, P. M. Root-knot nematode resistance in wild Arachis. In: CONGRESSO BRASILEIRO DE NEMATOLOGIA, 29., 2011, Brasília, DF. Anais... Brasília, DF: Sociedade Brasileira de Nematologia, 2011. p. 63-66. Palestras.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 19. | | PROITE, K.; LEAL-BERTIOLI, S. C. M.; BERTIOLI, D. J.; GUIMARÃES, P. M. Análise "in silico" da expressão gênica diferencial de Arachis stenosperma inoculado com nematóide das galhas. In: ENCONTRO DO TALENTO ESTUDANTIL DA EMBRAPA RECURSOS GENÉTICOS E BIOTECNOLOGIA, 10., 2005, Brasília, DF. Anais: resumos dos trabalhos. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2005. p. 56.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| Registros recuperados : 279 | |
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